Phospho irf3
WebPhospho-IRF3 (S386) and Total IRF3 (S386) ELISA Kit (ab279834) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated IRF3 protein in your experimental model system, you can verify pathway activation in your cell lysates. WebWestern blot analysis of extracts of NIH/3T3 cells, using Phospho-IRF3-S396 antibody (AP0623) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane.
Phospho irf3
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WebPhospho-IRF-3 (Ser396) Antibody detects endogenous levels of IRF-3 only when phosphorylated at Ser396. This antibody does not cross-react with other family members … Monoclonal Antibody for studying IRF-3 (Ser396) phosphate. Cited in 635 … WebApr 13, 2024 · Antibodies. The following antibodies were used: mouse monoclonal antibodies to α-tubulin (Santa Cruz, sc23948), GAPDH (Santa Cruz, sc32233), phospho-H2A.X (Ser139) (EMD Millipore, 05-636), anti ...
WebAll lanes : Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493) at 0.2 µg/ml (purified) Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates Lane 2 : … WebSep 1, 2024 · To systematically search for the phosphorylation sites on IRF3 in vivo, we carried out tandem affinity purification of recombinant double-tagged IRF3 expressed in HEK293 cells, coupling with mass spectrometric analysis (Fig. 1 A and B). Several phospho-peptides were identified with different color highlighted in the whole amino acid sequence …
WebIRF3 Antibodies Antibodies that detect IRF3 can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry and Peptide array. These antibodies target IRF3 in Human, Mouse, Rat, Non-human primate and Porcine samples. WebIRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response. In unstimulated cells, IRF-3 is present in the cytoplasm. …
WebPrimary Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859) Protein Recombinant Human IRF3 protein (ab205216) Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab205718) View more associated products Overview Product name Anti-IRF3 (phospho S396) antibody See all IRF3 primary antibodies Description
WebApr 23, 2024 · We focused on the phosphorylation of IRF3, which is a downstream factor of the cGAS pathway . As shown in Figure 2A, the expression of phosphorylated-IRF3 (p-IRF3) was increased in HNSCC cells after X-ray irradiation, and cGAS knockdown suppressed the radiation-increased p-IRF3. These results suggest that ionizing radiation activates IRF3 ... daily motion 04WebPhospho-IRF3 (Ser396) Antibody (BS-3195R) in WB Mouse liver lysates probed with Anti-IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:300 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37°C. Product Details Target Information dailymotion 090225cWebIRF-3 is a member of the interferon regulatory transcription factor (IRF) family. It mediates interferon-stimulated response element (ISRE) promoter activation and functions as a molecular switch for antiviral activity. DsRNA generated during the course of a viral infection leads to IRF-3 phosphorylation on the C-terminal serine/threonine cluster. biological warfare aiWebThe phospho-IRF3 (Ser386) assay measures IRF3 when phosphorylated at Ser386. Contrary to Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis or transfer. The phospho-IRF3 (Ser386) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. biological warfare in korean warWebDetect and quantitate cellular phospho-IRF3 (Ser386) using a homogeneous AlphaLISA® SureFire® Ultra™ assay. We are now two standalone organizations Life Sciences & … biological washing powder dog urine patioWebI'm using Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb #29047 from CST to detect phospho-IRF3 using both PMA-differentiated THP-1 and HT-29 cells treated with 2.5ug/mL LMW poly I:C by... dailymotion 1000 lb sistersIRF3 is a member of the interferon regulatory transcription factor (IRF) family. IRF3 was originally discovered as a homolog of IRF1 and IRF2. IRF3 has been further characterized and shown to contain several functional domains including a nuclear export signal, a DNA-binding domain, a C-terminal IRF association domain and several regulatory phosphorylation sites. IRF3 is found … biological warning symbol