Webexpression on a human myeloid cell line, U937 cells, following 45 hours exposure to the test chemical. The CD86 surface marker is a typical marker of U937 activation known to be a co-stimulatory molecule that may mimic DC activation, which plays a critical role in T-cell priming. The changes of CD86 cell surface Web1 aug. 2010 · We previously developed the human cell-line activation test (h-CLAT) in vitro skin sensitisation test, based on our reported finding that a 24-hour exposure of THP-1 cells (a human monocytic ...
DB-ALM Protocol n° 158 : human Cell Line Activation Test …
WebThese results suggest that our method, human Cell Line Activation Test (h-CLAT), using human cell lines THP-1 and U-937, but especially THP-1 cells at 24h treatment, may … WebBased on our findings, the protocol for this assay was optimized and the experimental conditions to be used in a future validation study were identified. We propose to call this kind of in vitro skin sensitization test h-CLAT, which is short for human Cell Line Activation Test. MeSH terms Allergens / toxicity* Antigens, Surface / analysis bram stoker s dracula book
EURL ECVAM Recommendation on the human Cell Line Activation Test …
Web30 jun. 2024 · This TG provides three in vitro test methods addressing the same Key Event on the AOP: (i) the human cell Line Activation Test or h-CLAT method, (ii) the U937 Cell Line Activation Test or U-SENS and (iii) the Interleukin-8 Reporter Gene Assay or IL-8 Luc assay. All of them are used for supporting the discrimination between skin sensitisers and ... Web30 jun. 2024 · The present Key Event based Test Guideline (TG) addresses the human health hazard endpoint skin sensitisation, following exposure to a test chemical. … Web18 jun. 2024 · To improve the accuracy of skin sensitization prediction of chemicals by conventional alternative methods using cells, it is important to reproduce the environment of skin in vitro, such as the crosstalk between keratinocytes and dendritic cells (DCs). We developed a skin sensitization test system based on the markers and criteria of the … brandi breakup